IL-6, IL-1β and TNF-α expression levels were measured to evaluate the inflammatory response in spinal cord tissue and cells. NLRP3, apoptosis-associated speck-like protein containing a CARD , pro-Caspase-1, Caspase-1, GSDMD, and IL-18 levels were measured to evaluate pyroptosis. Nrf2 and HO-1 levels were measured to assess the Nrf2/ HO-1 signaling axis. First, 100 mg of spinal cord tissue was washed with PBS, fully lysed with radioimmunoprecipitation assay buffer containing phenylmethylsulfonyl fluoride for 30 minutes, incubated on ice, and immediately centrifuged at 4°C. The supernatants were then collected, and the protein concentrations was determined using the bicinchoninic acid method with the PierceTM BCA Protein Assay Kit . The samples were loaded onto a polyacrylamide gel and electrophoresed. Next, the proteins were transferred to polyvinylidene fluoride membranes and blocked in 5% non-fat milk for 2 hours. Subsequently, the membranes were incubated overnight at 4°C with rat monoclonal primary antibodies against IL-6 , IL-1β , T N F - α , NLRP3 , ASC , pro-Caspase-1 , C a s p a s e - 1 , GSDMD , and IL-18 , followed by incubation with horseradish peroxidase–conjugated rabbit anti-rat IgG at 20°C for 1 hour, and finally stained with a diaminobenzidine solution. A Bio-Image gel imaging system was used to collect images. The optical density was measured using ImageJ software. Glyceraldehyde-3-phosphate dehydrogenase and β-actin were used as internal references.背景和对比度不应该加以修改以明显改变数据可见度、背景或非特异性带。
外泌体作为巨噬细胞与骨髓间充质干细胞之间沟通的“使者”,可通过传递miRNA、脂质、蛋白质等多种活性分子实现细胞间信息传递,其中miRNA作为外泌体的重要组成部分,受到外泌体脂质双分子层的包裹不易降解,从而更有利于miRNA的传递及进入目标细胞行使其调控功能。
复旦大学附属华山医院神经内科郁金泰教授团队联合复旦大学类脑智能科学与技术研究院的冯建峰/程炜团队发现了对阿尔茨海默病(Alzheimer’s disease,AD)诊断和预测具有重要价值的新型生物标志物——YWHAG,它在识别生物学定义的AD和临床诊断的AD痴呆时的准确度分别高达
杨晖正在进行实验操作【人物名片】姓名:杨晖职业:生物工程技术人员简介:一种生物科技和医学跨界融合的职业,用现代科技挖掘中华医药更多价值产业:生物医药顶端新闻·河南商报记者 邹皓羽/文 受访者供图深夜,河南省中医方证信号传导重点实验室里的灯还亮着,研究员杨晖在为攻克地龙多肽抗食管癌
关键词: 柚皮苷, miR-206, Cx43, ERK1, 成骨细胞, 骨损伤, 骨碎补, miRNAs。引用本文: 吴晶晶, 林海雄, 孙伟鹏, 李紫阁, 姜自伟. 柚皮苷调控成骨细胞成骨分化的机制. 中国组织工程研究, 2023, 27: 1722-1727.
大河网讯 “不安全行为一周后我失眠了,担忧、恐慌、不安接踵而来,悔不该当初没用‘安全套’和‘阻断药’。”李明(化名)对疾控中心艾滋病自愿咨询检测门诊(简称VCT门诊)医生说。关于艾滋病检测,河南省疾病预防控制中心艾防所专家耿杰进行了详细的科普。
日前,上海嘉定先进技术创新与育成中心(以下简称“育成中心”)生物技术创新非标实验室启用,首期投放11台生命科学仪器,助力生物医药企业科技成果转化。育成中心是一家专注于生物医疗产业领域的国家级科技企业孵化器。
关键词: 高糖, 血脑屏障, 紧密连接, 通透性, 细胞凋亡, 组蛋白去乙酰化酶4, 工程化组织构建。引用本文:杨洪涛, 许永劼, 周勇君, 王 双, 黄昶煜东, 朱丽英, 潘 卫, . 高糖对hCMEC/D3人脑微血管内皮细胞血脑屏障紧密连接的影响. 中国组织工程研究, 2025, 29: 5536-5542.
文题释义:树突棘:树突棘头部是2个神经元连接和交换信息的地方,包含神经元从其他细胞接收信息所需的所有成分,颈部可以非常薄,功能之一是通过调节物质进出头部的运动,维持神经元与神经网络中其他细胞之间的关系。